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At the end of incubation, 20 L of MTS reagent was added to each well and incubated for another two hours, then analyzed with a microplate reader using a test wavelength of 490 nm within 10 min

In the future, the development of lateral flow assay technology for detection of heavy metal ions will focus on multiplex detection, optimization of signal amplification strategies, integration with portable devices, and standardization and commercialization

The test can determine that it is a combination of muscular and brain creatine kinase that is present in the blood (CK-MB) This is evidence of damage to the heart