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The lysate loaded on to a 5 ml HisTrap HP column (Cytiva) equilibrated in lysis buffer and eluted with an imidazole gradient up to 100% elution buffer (50 mM HEPES, 500 mM NaCl, 0.5 mM TCEP, 500 mM imidazole, pH 7.5)
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Bile flow was significantly depressed in the TPN and EXP groups compared with FED controls (32.9 9.4 and 45.7 14.4 versus 82.9 13.8)
