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Add 50 L of Reaction Mix to each well

[1] [6] Severe deficiencies may include symptoms of reduced heart function, suppression of bone marrow, as well as more various severe neurological symptoms possibly including sensory loss, abnormal balance and reflexes, memory and consciousness impairment, smell and taste problems, depression, irritability, confusion, and psychosis

Garg R, Garcia-Manero G, Faderl S, Cortes J, Koller C, Beran M, Borthakur G , Kantarjian H, Ravandi F
Protective Effect of Naringin on DSS-Induced Ulcerative Colitis in Mice

Use of anionic denaturing detergents to purify insoluble proteins after overexpression