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Both strains were stored at 80C in Brucella Broth (BB, Thermo Fisher Scientific Remel Products, Lenexa, KS, United States) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (FBS, Gibco, Life Technologies, Carlsbad, CA, United States) and 20% (v/v) glycerol

Fontes Ribeiro C

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In addition, F-actin disorganization may alter localization and/or occlusive function of proteins forming the tight-junctional strands, such as occludin and claudins

Several cross-sectional studies have suggested that MMA is a potential risk factor for cardiovascular disease (CVD), although small sample sizes may have limited their effectiveness [24, 25]