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After PBS washing, sections were incubated with an HRP-conjugated, species-matched secondary antibody (50 min, room temperature), followed by further PBS washes

However, a direct reduction in the size of certain hypertrophic cardiomyocytes and replication of other size-reduced hypertrophic cardiomyocytes contribute significantly to the regression of copper-deficient cardiac hypertrophy, resulting in the normalization of the size and number of cardiomyocytes in the heart (113)