l carnitine hypertension L-Carnitine Side Effects & Risks Revisiting the Role of Carnitine

( b ) Body weight, fat mass and fat free mass at day 6

The cells were collected by centrifugation at 2000 g for 5 min and washed in phosphate buffered saline (8 gL 1 NaCl, 0.2 gL 1 KCl, 1.44 gL 1 Na 2 PO 4 , 0.24 g L 1 KH 2 PO 4 , at pH 7.5)

The remaining lamina propria tissue was further processed by incubating with a digestion solution containing collagenase (1 mg/mL) and DNase (0.2 mg/mL)

Yeast DNA Isolation and Yeast TransformationYeast chromosomal DNA was isolated by the glass bead lysis method and yeast transformations were carried out using the lithium acetate method (Gietz and Woods, 2002)

Szwed M, Marczak A