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Cells were washed once with PBS then once with HBSS and incubated for 30 minutes at room temperature with 50 nM TMRE (Invitrogen, Cat#T669), 1 M ER-Tracker TM green (Invitrogen, Cat# E34251) and 1 M Hoechst 33342 (Thermo Scientific, Cat# 62249) in HBSS covered from light

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While this study was not performed in the HCT conditioning setting, the depletion of healthy HSPCs and AML cells shows promise as a dual benefit in conditioning