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Briefly, after dissection the brain tissue was incubated for 10 min at 37 C in PBS containing trypsinEDTA (0.025%/0.01% w/v) and then mechanically dissociated at room temperature with a fire-polished Pasteur pipette
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In the cytosol formation of PSSG is transient except during oxidative stress
From this point on, we used Accutase solution to make single-cell suspension for further expansion of NPCs or terminal differentiation into neurons
UV protection prevents the breakdown of newly formed collagen and maintains the benefits of your hyaluronic acid application
