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HCYhomocysteine, Cys- cysteine were measured in uM

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Under oxidative stress, SIRT1 undergoes deacetylation as the NAD+/NADH ratio rises, which leads to the activation of PGC-1 [35]

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Briefly, determined the protein concentrations in each group using a detergent-compatible protein assay (5000002, Bio-Rad) and prepared equal quantities of protein (50 g) samples