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Micro slide 8-well glass bottom plates (Catalog 80807, Ibidi, Grfelfing, Germany) were treated with 40 L of a 1 mg/ml Concanavalin A (Catalog C2010, Sigma Aldrich, prepared in 10 mM Sodium phosphate buffer pH 6, 10 mM CaCl 2 ,1 mM MnCl 2 , 0.01% NaN 3 ,) solution, incubated for 5 min and then washed twice with 40 L of fresh medium
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Inject Bacteriostatic Water into BPC-157

doi:10.1093/aje/kwv135

To investigate the effects of Thr starvation death further, we next performed a screen to determine whether withdrawal of essential AAs beyond Arg and Leu would influence threonine deprivationassociated cell death, and we prepared media lacking Thr combined with 13 other AAs individually dropped out (Fig 3H and I)
