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To stimulate the cells, 50 ng ml 1 LPS ( Escherichia coli O111:B4, Sigma) and 10 ng ml 1 recombinant mouse IFN- (R&D Systems) were added to the media

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Conversely, microglial responses influence gut physiology and immune responses through bidirectional pathways, including the vagus nerve and immune mediators

The reaction can also be performed under dilute conditions (10 mM) and on gram scale (3.5 mmol)

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