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Membrane was incubated at room temperature in blocking solution (1% bovine serum albumin, 1% goat serum in 1PBS) for 1 h, followed by incubation in blocking solution containing appropriate dilution of primary antibody for growth hormone receptor (R&D System, MN, USA) or phospho-Jak2 (Tyr1007/1008, Cell Signaling Technology, Inc

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As with any medical treatment, its important to consult with a qualified healthcare professional to determine if B12 shots are suitable for your individual needs and to ensure safe administration.

For short-term storage of a few weeks, a cool, dark place away from direct sunlight or heat, such as a cupboard, is sufficient

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