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The actual treatment is relatively quick and straightforward

Cells plated in glass-bottom 96-well plates were treated for the indicated times and, after fixation with 4% paraformaldehyde, images were acquired using a high-content microscope (Operetta, Perkin-Elmer)

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Moreover, the circadian rhythm of peripheral tissue is responsive to the feedingfasting cycle [235].Timerestricted circadian feeding, which involves setting the feeding period to a defined interval without a reduction in caloric intake, is associated with systemic inflammation [236, 237]