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Several studies have demonstrated that NAD + metabolism is involved in neuronal function and in the pathophysiology of neurodegenerative diseases (Hikosaka et al., 2019) and that NAD + levels in tissues can produce beneficial therapeutical effects in this type of diseases (Kulikova et al., 2018)

Hair fibers were first added to concentrated sulfuric acid, sonicated for 30 min, and stirred for 24 h at 40, 100, or 140 C

However, injection carries its own risks (infection, sterility, injection site reactions)

Peripheral venous blood was collected in plain blood collection tubes after an overnight fast of 12 h after the last dose of medication for the estimation of haemoglobin, blood urea, serum creatinine, liver function test, lipid profile using standard techniques