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The PVDF membranes were incubated with primary antibodies overnight at 4 C, incubated with secondary antibodies at room temperature for 1 h, and visualized using the Bio-Rad ChemiDoc XRS + system
Zinc deficiency has been associated with an increased risk for several cancers [such as endometrial (369, 370), prostate (367, 368), pancreatic (368), breast (368), gynecological (371) and lung cancer (372)]
Alcohol depletes these minerals, so replenishing them is key
doi: 10.4161/cam.1.3.5114
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