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Moreover, fisetin has been shown to alleviate oxidative stress caused by transition metals, a major contributor to neurodegeneration in PD, by chelating metal ions and diminishing metalcatalyzed lipid peroxidation and protein aggregation [42]

Protein-lipid overlay assay After 24 h of transfection, N2a cells were washed twice with TBS (50 mM Tris-HCl, pH 8.0, 150 mM NaCl, and 10% glycerol) and cell extracts were prepared as explained above in TBS supplemented with 0.5% Tx-100 and protease inhibitors

Expression and prognostic significance of IAP-family genes in human cancers and leukemias

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