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Description
Following injection, the needle was left in place for 3 s before being withdrawn, and gentle pressure was applied to the injection site to prevent bleeding
Differential distribution of immunoreactive S100-alpha and S100-beta proteins in normal nonnervous human tissues

doi: 10.1210/endo.135.5.7525254

The problem is that physiological GH secretion is fundamentally pulsatile

Insert the needle using a smooth, steady motion until its fully under the skin
