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LC-MS/MS studies developed assays and confirmed detectability of metabolites in horse plasma/urine after administrationuseful as hard evidence of veterinary use, albeit in a doping-control context rather than therapy

Clearly, under normal conditions, Keap1 strictly regulates Nrf2 expression to a low level in order to avoid overexpression of its target genes [55]

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The blots were developed using SuperSignal West Pico (Thermo Scientific, 34-580), clarity enhanced chemiluminescence western blotting substrate (Bio-Rrad, 1-709-060) or Femto chemiluminescence substrate systems (Thermo Fisher Scientific, 34-094)