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Rampelt, H

For LC-MS, the reaction was prepared with 10 M enzyme, 200 M GTP in 20 mM HEPES buffer, pH 6.8, 150 mM NaCl, 10 mM MgCl 2 .The reaction was carried out at 30 C for 5 min, then deactivated at 95 C for 5 min

However, if untreated, a vitamin B12 deficiency can lead to serious symptoms, including nerve damage or pernicious anaemia a condition when your body doesnt have enough B12 to make the red blood cells it needs

Anecdotal evidence suggests that people who receive B12 injections before or after drinking report feeling more alert and less fatigued

Advisers to this series are Robin Ferner and Patricia McGettigan