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Description
Across several research papers, similar molar ratios of approximately 50:10:38.5:1.5 have been maintained for developing LNPs (Carrasco et al., 2021

doi: 10.1096/fj.10-165050

add 1ul of 1M Tris, pH8 (final 50 mM Tris)
doi: 10.3174/ng.2300055 39 GanjiS

Thus, miR-483-3p is a strong candidate repressor of the IGF-1 gene