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For Drosophila expression, the plasmids containing the constructs Go-Nt 7 -GFP, MGNC-Nt 7 -GFP, and MGSLCSR-Nt 7 -GFP were cut with EcoRI/NotI and inserts were ligated into the same sites of pAc5.1/V5-HisA (Thermo Fisher Scientific, V411020)

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Nogueira GB, Punaro GR, Oliveira CS, Maciel FR, Fernandes TO, Lima DY

This systematic approach ensures research quality, enables meaningful comparison with published literature, and contributes to the evidence base that may eventually support regulatory evaluation of BPC-157 for potential therapeutic applications

However, the SVM model was marginally better than the DNN model for chemicals with a basic pKa