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Western immunoblot analysis The serum samples and liver homogenates (previously prepared for determination of oxidative stress biomarkers) were separated by 12% SDS-PAGE and transferred onto polyvinylidene difluoride membranes (Hybond-P, Amersham Pharmacia Biotech), blocked in a 0.2% Tween 20, 50 mM Tris HCl pH 7.6, and 150 mM NaCl solution containing 5% non-fat condensed milk

Its also available as a dietary supplement sold in health stores or online
6 g twice a day) increased plasma -hydroxybutyrate concentration from ~ 0.1 mmol/L to ~ 0.2 mmol/L [32]
[DOI] [PMC free article] [PubMed] [Google Scholar] 12.Thakur N, Raigond P, Singh Y, Mishra T, Singh B, Lal MK, Dutt S
