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This last point is speculative

For example, in a resting state, glycogen synthase kinase-3 (GSK-3) phosphorylates NRF2, creating a site recognized by the E3 ligase adapter beta-transducin repeat-containing protein (-TrCP)

doi: 10.1016/j.fct.2011.11.014 44 GuoQ.ShenZ.YuH.LuG.YuY.LiuX.et al

Now that we are done with our purification and intentionally stripping the nickel ions off of the column, however, it is useful to use 50 mM EDTA to get all of the nickel out of there

Nevertheless, we cannot rule out the possibility that when dose, route, and frequency of administration are altered, another one of our peptides may become more beneficial than sB