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In vitro splitting assay The purified RNCs (100 nM ribosome) containing 100 nM Rqc2 were incubated with or without 10 nM RQT complex and 1 mM ATP in reaction buffer (50 mM HEPES, pH 7.4, 100 mM KOAc, 2.5 mM Mg(OAc) 2 , 0.01% NP-40, 5 mM -mercaptoethanol, and 0.2 U/l SUPERase In RNase inhibitor) for 45 min at 25 C

58 (2): p

While extensive research has been conducted on lung IRI using other established animal and cell culture models, the underlying mechanisms and signal transduction pathways remain elusive, and a specific, effective treatment is still unavailable

However, these findings should be cautiously interpreted, as the available evidence is constrained by methodological limitations

However, GlaxoSmithKline voluntarily withdrew albiglutide from the market in 2018 for economic reasons