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Cells were then harvested for RNA/protein extraction and subsequent analysis by RT-PCR, WB, and flow cytometry
Netrin-1 mediates nerve innervation and angiogenesis leading to discogenic pain
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N -Methyl-D-aspartic acid (NMDA) is -amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) were blocked by 10 M (RS)-CPP (Tocris) and 10 M NBQX (Tocris), respectively
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