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electrochemiluminescence detection of each MSD SULFO-TAG was captured and quantified using SECTOR Imager instrumentation (MSD, Gaithersburg, MD, USA), according to manufacturers protocols as reported previously ( The raw data were analyzed using the Discovery Workbench 4.0 software (MSD) by fitting signal from the control calibration curves to a four-parameter logistic model and then back-fitting the electrochemiluminescence signal from each sample to calculate the unknown concentration

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For instance, the regulation of mitochondrial proteins and biogenesis has been connected to miRNAs, including miR-125b-5p, miR-132-3p, miR-19a-3p, miR-30a-5p, and miR-660-5p, which exhibit changed expression levels in the follicular fluid of women with reduced ovarian reserve

To assess in vivo tumor growth potential PRX3 KO and control cells were implanted in the peritoneal cavity of immunocompromised mice and end-stage tumor burden was determined after 41 days of growth

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