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Primary antibodies were added, and the cells were incubated for 2 h at 37 C after washing and blocking for 30 min

Pro-apoptotic elements, BCL2 associated X, apoptosis regulator (BAX) and BCL2 antagonist/killer 1 (BAK1), drive processes such as mitochondrial outer membrane permeabilization and the creation of the mitochondrial permeability transition pore (MPTP), thus regulating the formation of pores in the outer mitochondrial membrane [112]

However, the main anti-fibrotic therapeutic effect is due to a regenerative effect, which is mainly driven by the ASCs, either extracted and expanded in culture or as they are in the lipoaspirate or SVF (Figure 8 (2))

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