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The following day, media was removed and cells were washed with PBS and 500 l of fresh RPMI was added containing 2P-AC (1 mM and 4 mM dissolved in water) before infecting the cells with Mtb at MOI of 5:1 (bacteria:macrophage)

The location of the SMHT1 gene (17p11.2) resides within the 3.7 Mb region of chromosome 17 that is deleted in Smith-Magenis syndrome, SMS, a disorder associated with behavioral problems, psychomotor impairment, growth delay, speech delay, brachycephaly, midface hypoplasia, and a hoarse voice

While the proportion of patients with low plasma vitamin B12 levels did not significantly differ between the two groups, elevated plasma homocysteine is a more sensitive marker of vitamin B12 deficiency

Wree A, McGeough MD, Pena CA, Schlattjan M, Li HY, Inzaugarat ME, et al