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Precipitating factors of HE include gastrointestinal bleeding, infection, constipation, infection, excessive intake of protein, dehydration, renal function disorder, electrolyte imbalance, psychoactive medication, and acute hepatic injury

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Subsequently, 10 l of the reaction mixture was combined with 10 l of 0.01 mM D9 pentanoyl-CoA as an internal standard (IS), followed by mixing with 50 l of 200 mM formic acid to terminate the reaction

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