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Cells were cultured in mTeSR1 medium on 100-fold-diluted Matrigel matrix and routinely passaged using Accutase [1]

The first is characterized by reduced hepatic IGF-1 production following GH stimulation, with a reduction of the effects of IGF-1 on growth, anabolism, and feedback regulation of GH
Many people do both, but it is worth a quick chat with your provider first

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Zhu Y, Wang H, Fang J, Dai W, Zhou J, Wang X, et al
