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Autophagy 12 (8), 14251428

Swarm edges were examined with a Nikon Inverted TE200 microscope (Nikon, Melville, NY) using a 10 objective, and digital images were collected with a SPOT RT camera and SPOT Basic software (Diagnostic Instruments, Inc., Sterling Heights, MI)

After transferring and blocking to nitrocellulose membranes, the blots were incubated overnight with primary antibodies against the target protein, at 4 C

These individuals often need larger doses of B12 to fulfill the important roles B12 has in human physiology