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First, hydrochloric acid in the stomach separates vitamin B12 from the attached protein

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To analyse reduced and oxidized PDI by western blot, cells were lysed in 100 mM Tris-HCl pH 6.8, 2% SDS supplemented with PhosSTOP and cOmplete protease inhibitor cocktail and 40 mM ethylmaleimide to block free cysteines

The euthanized mice were wrapped in foil and kept frozen at -76 C until they were returned to Earth
