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In research contexts each compound is titrated independently and slowly, and neither is pushed to its maximum simultaneously

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Al-Hetty HRAK, Saleh MM, Jalil AT

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Lentiviral vector production and cell transduction Transfection mixture was prepared in 800 L OptiMEM (Gibco) by adding following plasmids: 12 g of psPAX2 (AddGene #12260), 5 g of pVSV-G (AddGene #138479) and 12 g of expression vector carrying shRNA sequence, in the presence of 80 L of TransIT- LT1 Transfection Reagent (Mirus, Mir2360)