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Lon protease binds PYCR1, a key enzyme in proline metabolism, enhancing NADPH consumption and promoting electron leakage in the ETC, thereby elevating mtROS (45)

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Furthermore, we scored each cell for expression of human liver-enriched genes 6 and found that KO cells were enriched for these genes compared to WT cells, with the emphasis on MLP2, ML, and surprisingly, endocrine cell clusters (Fig