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Insulinotropic glucagon-like peptide I receptor expression in glucagon-producing alpha-cells of the rat endocrine pancreas

Furthermore, heightened levels of ROS and reactive nitrogen species generated by mitochondria can initiate oxidative and nitrosative stress, exacerbating damage to both mitochondria and cardiomyocytes (39)

Int J Biol Macromol 163:603617

16, 2004 BioWorld Science Endocrine/metabolic

The procedure was as follows: equilibration of the affinity gel as a batch using a glass filter funnel with binding/washing buffer consisting of 10 mM TrisHCl pH 7.8, 0.2 M NaCl, 0.2 mM dithiothreitol (DTT) and 1 mM EDTA