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PMID: 26029106

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The proteins were embedded in a box of OPC water molecules (minimum distance of 12 from the solute to the boundaries of the box) and the net charge of the system was set to zero by neutralization with Cl-ions using the program tLEaP included in AmberTools21 ( 60

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By keeping the solution refrigerated, you are essentially putting the brakes on these molecular interactions, preserving the integrity of the entire system for as long as possible