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Ossein Hydroxyapatite Complex Source of natural bone-growth fact

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Protein quantification was conducted using a BCA protein assay kit (Beyotime, Shanghai, China), and 50 micrograms of protein were heated at 95 C for 5 min with a quarter volume of SDSPAGE sample loading buffer

Source and gas setting were as follow: curtain gas = 30, CAD gas = medium, ion spray voltage = 5.5 kV in positive mode and 4.5 kV in negative mode, temperature = 550 C, nebulizing gas = 50 and heater gas = 60