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Both point mutations and alanine substitution of the serine and threonine residues from 375 to 379 (STANT sequence) has been indicated to markedly decrease the agonist-induced recruitment of -arrestin and receptor internalisation

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In shuttle-saturated cells, reduced flux of pyruvate through the pyruvate dehydrogenase complex and the TCA cycle means less citrate is generated and exported from mitochondria