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Description
The column was washed with lysis buffer (5 mM imodazole in lysis buffer) and increasing concentrations of NaCl (0.5 mM and 1 mM) followed by increasing concentrations of imidazole (5 mM and 20 mM, in lysis buffer) to remove contaminating proteins
Phase IIb/III trials were conducted for obesity but fell short of the primary endpoint
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The standard cycle consists of 8 weeks on treatment followed by 4 weeks off
