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The lysed cells were sonicated and centrifuged at 14,000 g for 15 minutes to sediment insoluble cell debris

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For instance, high-mobility group box-1 (HMGB1) expression was found up-regulated in response to METH treatment and shown to mediate the neuroin?ammatory response in the nucleus accumbens, ventral tegmental, and prefrontal cortex of the brain (Thomas et al., 2008)