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Cells were cultured in Dulbeccos modified Eagles medium + GlutaMAX (GIBCO, 10566-016), supplemented with 10% of Foetal Bovine Serum (FBS) and 1% penicillin/streptomycin PenStrep (Euroclone, ECB3001D)

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After administration to dietinduced obese mice, the REVERB agonist resulted in increased energy expenditure, decreased obesity, and improved dyslipidemia and hyperglycemia [222]
