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Cell lysis for protein preparation The yeast cell pellet in a 1.5-mL tube (on ice) was resuspended with 500 L ice-cold lysis buffer (20 mM Tris-HCl pH 7.5, 150 mM NaCl, 1.8 mM MgCl 2 , 0.5% NP-40, 1 mM PMSF, 1 mM DTT, 20 M MG132, 1 tablet/10 mL cOmplete mini EDTA-free (Roche)) and transferred to a new 1.5-mL tube containing 50 L of 0.5 mm Zirconia/Slica Beads (BioSpec Products)
Free-radical and biochemical reactions involving polar part of glycerophospholipids
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