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K-means clustering analysis of amplicon sequence variants by animal demonstrated that NNMTi-treated DIO mice switched to LD had a distinct microbiome pattern that was highlighted by decreased Erysipelatoclostridium and increased Lactobacillus relative abundances compared to vehicle counterparts

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Both in vivo and in vitro experiments show IGF-2 activates the IGF-1R/PI3K/AKT pathway, reducing apoptosis and inflammation