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III Isobaric labeling-based relative quantification in shotgun proteomics

[PMID: 24283226 DOI: 10.1056/NEJMra1208707] [DOI] [PubMed] [Google Scholar] 44.Griffiths MJD, Evans TW

Natl Compr

Equal amounts of proteins from lysates were separated by SDSPAGE using Mini-PROTEAN TGX 10% Precast Gels (Bio-Rad, Hercules, CA, USA) before transfer onto a Sequi-Blot PVDF membrane (Bio-Rad) using the Mini Trans-Blot Electrophoretic Transfer Cell system (Bio-Rad)

S -adenosyl methionine (SAM) is the universal methyl donor in energy metabolism and is produced from methionine by the methyltransferase MAT2A
