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LC-MS/MS Analysis With DDA Samples were injected (30 L) onto an Aeris PEPTIDE XB-C18 100 2.1 mm column, with solid core 1.7 m particles (100 ) fitted with a SecurityGuard ULTRA C18-peptide guard column (Phenomenex, Torrance, CA) using a Nexera UHPLC system (Shimadzu, Columbia, MD) with water (A) and ACN (B), both containing 0.1% formic acid, at a flow rate of 0.3 mL/min (40C)
Something to consider when evaluating whether or not to cleave the GST moiety away is that GST is a homodimeric protein
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Here, excitatory glutamatergic neurons project to the GPi
Studies have consistently shown that pulse intravenous methylprednisolone is superior to oral glucocorticoids both in terms of efficacy and decreased side effects for managing Graves' orbitopathy