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NNMT is overexpressed in white adipose tissue during obesity, in senescent muscle stem cells during aging, and in certain cancer types

Then, lysis buffer (1% NP-40, 10% Glycerol, 137 mM NaCl, 20 mM Tris pH7.6, 20 mM NaF, 2 g/mL aprotinin, 2 g/mL leupeptin, 2 g/mL pepstatin, 200 M Na3VO4, 1 mM PMSF) was added and cells were stored at 4 C for 30 min

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For statistical comparison, analysis of variance (ANOVA) or Students t tests with post hoc testing, Tukeys multiple comparison test and MannWhitney test two-tailed for parametric and nonparametric data, were used

B12 is involved in so many processes