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Description
Drug transport in the liver
doi: 10.1111/j.1749-6632.1999.tb07866.x, 74
The resulting supernatant was layered over a 1-mL sucrose cushion (20 mM HEPES/KOH pH 7.5, 150 mM KCl, 10 mM MgCl, 1 M sucrose) in a 3.5-mL thick-wall polypropylene centrifuge tube (Beckman, #349623) and centrifuged at 250,000 g for 4 h at 4 C in a Beckman TL100 ultracentrifuge (TLA 100.3 rotor)
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