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After incubation with collagenase IV, cells were separated by mechanical destruction of tissues, filtered through a 100 m cell filter, and then, centrifuged at 700 rpm for 2 minutes to obtain cell precipitates

In breast cancer, CHAC1 overexpression promotes both proliferation and migration, while in ovarian cancer, its impact is primarily restricted to cell migration, with minimal effect on proliferation ( TP53, encoded by the TP53 gene and mutated in over 50% of human cancers, is essential for cell-cycle regulation and the induction of programmed cell death