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General protein purification buffer considerations Buffer components to limit or avoid entirely For GoldBios His-Tag Buffer Set and His-Tag Column Prep Protocol, we recommend using 50 mM Phosphate Buffer pH 8, 300 mM NaCl, and 10 mM Imidazole as the binding buffer, and this is a great baseline buffer to use if youre working with a new protein and unsure what is important to include in your purification buffers

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Further studies showed that crosstalk between hypoxia and this pathway is mediated through HIF-1 (160)

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20, (Suppl.) 147S152S