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Tufail M, Jiang C-H, Li N

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Cells were grown to ~70% confluency before treatment for 24 hours with various concentrations of bovine intestinal alkaline phosphatase (Sigma: A2356) in the absence or presence of bafilomycin A1, an autophagy flux inhibitor (100 nM, added for 3 hrs towards the end of 24 hr incubation.) (Sigma: SML1661)

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With the application of omics technologies, a systems biology approach [219] further provides a dynamic, multi-scaled, and whole-body approach to understanding ASD and the development of personalized therapeutics